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Peer review Peer review information Nature Reviews Rheumatology thanks C

Mice from each of the age groups were utilized within an experimental period

After 30 minutes at 37C, 500 L of cold ethanol was added to the homogenate to precipitate proteins, followed by centrifugation (21,000 g , 4C, 10 minutes), and the supernatant was dried under an N 2 stream at room temperature

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high copper intake downregulates intestinal absorption and upregulates biliary excretion, whereas deficiency states promote intestinal uptake and reduce biliary loss to conserve copper [89]