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Organoid preparation and culture To generate gastric cancer organoids, fresh tumor tissues were transported on ice in cold DMEM/F12 (Gibco) with primocin (InvivoGen), then washed in cold PBS, minced into fragments (1 mm), and digested for 1 h at 37 C with collagenase and hyaluronidase

First, researchers should assess whether their primary hypothesis involves isolated collagen induction or comprehensive tissue remodeling this single question typically resolves the Matrixyl vs GHK-Cu selection in most study designs

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