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For the assessment of kidney injury severity (severity score), HE stained section images at 10 and 20 (2 at each magnification, for a total of 4 images per mouse) were graded from 0 (no damage) to 5 (most severe damage observed across the samples analyzed), based on the following parameters: (i) protein cast formation, (ii) visible tubular dilation, (iii) loss of proximal tubular cell polarization (no visible brush-border membranes) (iv) area affected (with single nephron damage to majority of the cortex with visual signs of injury)

Suggested Charge Rate: Up to 2C

46(5): 7279 Chace DH, DiPerna JC, Mitchell BL, et al

BMI body mass index WAT white adipose tissue BAT brown adipose tissue UCP1 uncoupling protein 1 NF-B nuclear factor kappa-B IL-6 interleukin-6 LPS Lipopolysaccharides CD14 cluster of differentiation 14 BAs bile acids HFD high-fat diet GM Gut Microbes PGC1 Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1- CPT1 carnitine palmitoyltransferase 1 Rb Retinoblastoma P38 MAPK P38 mitogen-activated protein kinase PPAR Peroxisome Proliferator-Activated Receptor CEBP- CCAAT Enhancer-Binding Protein IL-1 Interleukin-1 IEC intestinal epithelial cells Wnt Wingless/Integrated CEBP- CCAT/enhancer-binding protein TRAF4 TNF receptor-associated factor 4 CARM1 histone arginine methyltransferase 4 AMPK AMP-activated protein kinase ACC2 Acetyl-CoA carboxylase 2 STAT3 transcriptional activator 3 FOXO1 Forkhead box O1 GLUT4 Glucose Transporter 4 TG Triglyceride HO-1 Heme Oxygenase-1 TNF- Tumor Necrosis Factor-alpha LFD low-fat diet Glossary References 1

KE-0254/155/2014