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Integration of microbiology, molecular pathology, and epidemiology: a new paradigm to explore the pathogenesis of microbiome-driven neoplasms

However, gaps remain in the understanding of subset functions and regulatory mechanisms: in vitro studies rely on limited cell lines (e.g., HD11 macrophages, DF-1 fibroblasts), and the lack of long-term primary immune cell culture systems hinders simulation of in vivo cellular functions in complex microenvironments

The colonies were inoculated into 200 L YP20D medium containing 14 mM of acrolein, and cultivated at 30 C and 900 rpm for 48 h