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43 Statistical analyses Within each region, biomarkers were evaluated using a multiple linear regression to test the group difference between cases and controls

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RNA sequencing and data analysis RNA integrity was checked using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA)

Moreover, another study has shown that gut microbiota depletion with antibiotics improved the survival of the mice bearing tumour cells [196]