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RT-qPCR and WB experiments demonstrated that, in addition to ferritin, NAC also helped mitigate the effects of CGA on cellular processes in L-02 cells (Figure 7E

While no significant differences in anxiety and depression scores were observed between groups, the study emphasized the need for further research with larger sample sizes and optimized supplementation protocols [242]

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Moreover, we discovered that USP2 OE, but not its K447R mutant, led to a decrease in GPX4 mRNA level and a reduction in both GSH and SOD levels, while simultaneously causing an upregulation in ACSL4 mRNA levels and an increase in MDA content under TGF-1 stimulation (Fig