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Hydroxyl radical oxidation of guanosine 5-triphosphate (GTP): requirement for a GTP-Cu(II) complex

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Rapid ligand-receptor binding and efficient uptake by target cells are critical for receptor-targeted delivery systems to avoid in vivo clearance

coli 0111:B4 strain (Alexis, Enzo Life Sciences, Farmingdale, NY, USA), 1000 U/ml G-CSF (Myelostim, Italfarmaco Spa, Milano, Italy) and then plated either in 6/24-well tissue culture plates or in polystyrene flasks (from Greiner Bio-One, Kremsmnster, Austria) for culture at 37C, 5% CO 2 atmosphere