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The main precautionary note for GHK-Cu concerns individuals with known copper metabolism disorders or Wilsons disease, for whom any additional copper loading, including from GHK-Cu, would be contraindicated

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Chronic treatment requirements further complicate therapy, particularly in elderly patients with multiple comorbidities who are highly susceptible to adverse drug reactions and drugdrug interactions (Singh et al., 2024)

After incubation, the wells were gently washed twice with phosphate-buffered saline (PBS), and the cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes

Blood brain barrier receptor antibodies and methods of use