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The body's GH axis evolved to operate in pulses because continuous GH receptor stimulation leads to receptor downregulation and desensitisation

Results A counter-selection system was engineered in Saccharomyces cerevisiae based on the known feedback inhibition of gamma-glutamylcysteine synthetase ( GSH1 ) gene expression, which is rate limiting for GSH synthesis: the GSH1 ORF and the counter-selectable marker GAP1 were expressed under control of the TEF1 and GSH-regulated GSH1 promoters, respectively

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The formula of Livak and Schmittgen [21] was used for quantification of samples and normalization was per- formed using the housekeeping gene YWHAZ