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This creates a restorative period where energy demands are lower, allowing for clearance of metabolic waste like ROS and replenishment of energy stores (Ramanathan et al., 2002

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The kinetics of the aminoacylation reaction can also be used as a distinctive mechanistic signature, as aminoacyl-tRNA release is the rate limiting step for class I enzymes (except for IleRS and some GluRS) while for class II it is the amino acid activation rate instead (Fersht 1977