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CM-DCF fluorescence inside cells was measured at 485 nm for excitation and 538 nm for emission with FlexStation 3 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA)

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BBB breakdown can lead to neurotoxic accumulations of fibrin, thrombin, and plasmin, and red blood cell extravasation, the release of hemoglobin and ferrous iron, causing reactive oxygen species (ROS), which can all injure dopaminergic neurons [513]

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