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For histone analysis, cell pellets were acid-extracted overnight using Abcams histone extraction protocol in phosphate-buffered saline containing 0.5% Triton X-100, 2 mM PMSF, 0.02% NaN 3 , and 10 mM sodium butyrate and supplemented with cOmplete Protease Inhibitor Cocktail before use

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95 In essence, anti-inflammatory, pro-resolution, and antimicrobial activities of IL-6 are facilitated by classical signalling, whilst pro-inflammatory activities of IL-6 are facilitated by trans-signalling

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This stage is followed by damage to alveolar epithelial cells