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The cultured SGNs from P3 WT mice were incubated with 100 M peroxynitrite for 48 h with or without the pretreatment of 30 M ebselen for 1 h, or the SGNs from gpx1 / mice were cultured and exposed to 100 M peroxynitrite for 48 h

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The tissue was placed in an Eppendorf tube with 500 l of ice-cold homogenization buffer containing 1xPBS pH 7.2, supplemented with a protease inhibitor (complete EDTA-free protease inhibitor cocktail, Roche, Germany), 0.05 Sodium Azide and 0.5% Triton-X-100