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Phosphorylated MLKL undergoes oligomerization, a critical step for the translocation of MLKL to cellular membranes, culminating in membrane permeabilization and necroptosis 61

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Immunofluorescence staining in vitro T3M4 cells and PSC were seeded in gloss bottom dishes and received treatments of different formulations containing 20 M (T3M4 cells) or 10 M (PSC) of TPCA-1 and 50 nM of AsiG for 48 h at pH 6.5, followed by fixation in 4% paraformaldehyde for 10 min at room temperature

Representative traces of Vm/CaT recording with 10 mM BAPTA in the patch pipette under 300 nM Iso stimulation at 1-3 Hz pacing

This network model provides a theoretical basis for the high recurrence rates following hormone monotherapy or surgery