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NAD+ plays crucial roles in maintaining genomic stability through its function as a substrate for poly(ADP-ribose) polymerases (PARPs), a family of enzymes that detect and respond to DNA damage by catalyzing the synthesis of poly(ADP-ribose) polymers from NAD+

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CPA digestion of albumin To initiate in vitro enzymatic cleavage by CPA, unfused albumin (WT or QMP) or albumin fused to GLP-1, FIX 9 , or ACE2 13 (15 M), in DPBS (pH 7.4) (Sigma-Aldrich), were incubated with 30 g/mL of human pancreatic CPA (Elastin Products Company, Inc.) for 5 h at 37 C on a shaker platform set to 450 RPM