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16 Factors like ROS and impaired antioxidant systems can accelerate the process by damaging melanocytes in the outer root sheath and bulb, disrupting the renewal and migration of MSCs

The primary antibodies used were mouse anti-human GSS (1:1000, Abcam, ab5513), mouse anti-human GSR (1:500, Abcam, ab55075), and rabbit anti-human glutamate-cysteine ligase-catalytic subunit (GCLC

Additionally, treatment with NAC + oe-VWF led to decreased VWF expression, levels of IL-6 and TNF-, and protein levels of Collagen I and -SMA as compared to treatment with oe-VWF alone (Fig