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Immunocytochemistry and quantification of epSPC-neurospheres cultures Before the fixation, cells were washed with PBS for three times and then were fixed using 4% paraformaldehyde (Sigma) in PBS pH 7.4 for 15 min at room temperature (RT)

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UK clinical guidelines explicitly recommend cyanocobalamin as a safe, cost-effective form of B12, though other chemical forms like methylcobalamin are also effective [12] [13]

Summary Vitamin B 12 (cobalamin) plays an essential role in enzymatic reactions responsible for red blood cell (RBC) formation and myelination of the nervous system