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Accordingly, in human coronary artery endothelial cells (HCAECs) in vitro, Trx overexpression protected against hypoxia/reoxygenation-induced eNOS glutathionylation and preserved eNOS activity, whereas Trx silencing resulted in increased eNOS S-glutathionylation and uncoupling [163]

Additionally, citrate-bound Mn (about 2% of plasma Mn) may enter the brain via a shared citrate transporter, as its influx exceeded diffusion and the transporter was competitively inhibited by Mn citrate ( Intracellularly, both DMT1 (Wolff et al., 2018) and mitochondrial calcium uniporter (Kamer et al., 2018) can participate in Mn 2+ uptake into the mitochondria in vitro (Figure 1D)

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