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10-20 liters: Short outing or beach trip with one or two people 20-40 liters: Full day event or tailgate with a small group of people 40-60 liters: Weekend camping trips for a small group of people 60+ liters: Multi-day camping trips and other extended trips for a mid-sized group of people Some hard coolers are more durable than others

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Total protein was filtered with a 0.22-m membrane (Millipore, Corp., Billerica, MA, USA), and further purification with the kta Explorer 10 protein purification System (GE Healthcare, Piscataway, NJ, USA) used an ion-exchange chromatography column (HP Q HiTrapTM 5 ml, GE Healthcare, Uppsala, Sweden), loading buffer was buffer A, and elution buffer was buffer B (50 mM Tris-HCl, 1 M NaCl)