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Materials and methods Heart tissue homogenates In this study, remaining cardiac tissue homogenates obtained under the National Science Centre Poland project number 2019/33/N/NZ3/01649 approved by the Ethics Committee for Experiments on Animals at the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wroclaw, Poland (Resolution 002/2020 of 15th January 2020) were used

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N-Acetyl-L-Cysteine (NAC) is an amino acid that is a precursor to glutathione, the bodys most powerful antioxidant, which helps support optimal liver detoxification, lung health, antioxidant status, and muscle recovery

The isolated cells are reliably reproducible, and the drug doses used are much smaller and suitable for large-scale screening systems (6)