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For surface staining, cells were stained with the indicated antibodies (1:100 dilution) at room temperature (2025 C) in the dark for 30 min and then labeled with Fixable Viability Dye eFluor 520 (65-0867-14, eBioscience) at room temperature (2025 C) in the dark for another 15 min

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Now the FDA says Zyn also can market 20 of its pouches as safer than cigarettes, authorizing it to claim that the product puts you at a lower risk of mouth cancer, heart disease, lung cancer, stroke, emphysema, and chronic bronchitis. The move is rattling some health organizations, with Lisa Licasse, president of the American Cancer Society Cancer Action Network, warning about its potential effects for young people