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Cellular senescence: the good, the bad and the unknown

Breastfeeding is not contraindicated during LCT restriction, but should be complemented with MCT-containing formula [52, 143]

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2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)