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GLP-1 therapy works in significant part by weakening the body's normal appetite feedback, the homeostatic system that increases hunger as weight is lost

Improved fat oxidation helps mobilize stored adipose tissue for energy, contributing to sustained fat loss while preserving lean tissue

All cell lines, except Jurkat and THP-1, were maintained in DMEM high glucose (4.5 g l 1 glucose, 21969-035, Gibco) supplemented with 10% fetal bovine serum (FBS), 2 mM l-glutamine, and 1% penicillin/streptomycin at 37 C with 5% CO 2 , unless stated otherwise

reversing them takes months

Moreover, in experiments with AT 1 -mediated IP accumulation, Ang A produced a similar maximal effect as Ang II, indicating that it is a full AT 1 receptor agonist