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the spiked test solution as well as the control sample were filled into 3cc type I glass vials and were incubated at 37C for 24 hours

Mass spectrometry (MS) is a widely used analytical technique in metabolomics that allows for the identification and quantification of metabolites

Additionally, a cross-sectional study revealed that MMP9 levels significantly increased in obese PCOS patients and positively correlated with the duration of infertility [39]

Associations between MTHFR C677T and MS vary across populations, some case-control studies identify risk signals, while others do not, supporting a 'substrate-limitation' model where genetic predisposition interacts with B-vitamin status to constrain DNMT activity and stabilize a proinflammatory methylation landscape (7,142,143)

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