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Protein lysis and LEGENDplex assay Proteins from CuCl 2 -treated HepG2 and HepG2 ATP7B-KO cells were lysed by plating 500,000 cells in 800 l medium in 12-well plates

Husain, S.A

The supernatant was stored at 80 C and the total protein concentration was estimated according to the manufacturers instructions using a Bradford protein assay kit (Generay, China) (Jin et al

While novel drug targets for chronic disease are eagerly sought, potentially helpful agents that aid in detoxification of toxic elements, chelators, have largely been restricted to overt acute poisoning