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Ottanelli and colleagues [16] have shown that eosinophilic inflammation of the airway wall may affect the perception of dyspnea positively and that the association of eosinophils with inhaled corticosteroids may result in the perception of dyspnea in asthma getting worse (Figure 4)

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Subsequently, 150 L of 2 M Fluo-4 AM in serum-free DMEM medium was introduced, and the cells were incubated in darkness at 37 C for 60 min

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