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After appropriate mixing, samples were centrifuged and cultures prepared by spreading 0.1 mL of each sample on different media and incubated for 4872 hours at 37 o C

Those initial investigations were followed by others at The Wistar Institute and Penn Vet, in which viruses, less-pathogenic than SARS-CoV-2, were modified to express the SARS-CoV-2 spike protein

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