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In contrast, while CS extract reduced epithelial barrier integrity (determined by the translocation of dextran from the apical to the basolateral side of the cell layer), e-cigarette aerosol did not, suggesting that only CS extract negatively affected host defence [28]

Total daily nicotine uptake Since humans differ widely with respect to the metabolism of nicotine to cotinine and subsequent elimination of cotinine, the use of cotinine as a marker of nicotine uptake has been questioned

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