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MAX DNA polymerase (Doctor Protein Inc., Korea), 10 pmol of each primer, and 1 L of template were used for amplifications in a 25 L reaction volume

4 In parallel, the 5 As frameworkAsk, Advise, Assess, Assis, and Arrangeprovides a systematic approach to guide conversations around nicotine use, assess motivation to quit, initiate counseling and ensure appropriate follow-up

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