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ProActin5c::K2:TEV for cell culture transfection and transformation of flies contains DHFR T39A,E173D , amplified from K2:TTG1 using primers 36/37 ( Phe-K2 ) or 36/38 ( Met-K2 )

The complexity of dietary patterns and the presence of various confounding factors further complicate the interpretation of these results

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Chemiluminescent signal was read using a ChemiDoc system (BioRad #1708265), and the expression of bands of the target protein for each animal was expressed as % of -actin loading control

[1] Heterozygous women are usually normal because of lyonization ( X innactivation) [2] G6PD B, is the wild type or normal