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After TMB substrate solution is added, only those wells that contain Human SP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

Protect the vial from exposure to light and heat

Cell Biol Toxicol 37 (1), 5164

Furthermore, it should be noted that currently there is no agreed definition of what low, normal or high NAD+ levels are

The reaction yields a colorimetric signal where samples with reduced amounts of SOD emit a lesser signal that can be measured via colorimetry ( Catalase CAT is quantified by adding hydrogen peroxide (H 2 O 2 ) to the sample and analyzing the rate of decomposition of CAT, which is proportional to a reduction in the absorbance reading generated by the instrument ( Reduced Glutathione GSH can be determined by adding 5,5-dithiobis-2-nitrobenzoic acid (Ellman's reagent) to the sample