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The first instar larvae taken 24 h AEL (after egg laying), was orally administered with 2.5 mM Sp and fluorescence measurement in brains of eclosed adults were performed as mentioned above

10.1038/s41467-018-04310-9 74

Moreover, the fact that the two mutants, which have opposite effects in cell free assays (see above), are expressed at similar levels in hippocampal synapses, allows a stringent comparison in situ