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After washing with PBS containing 0.05% Tween20, the cells were treated with Alexa Fluor 555-conjugated anti-mouse IgG (Cell Signaling Technology)

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The pro-ferroptotic effect of CSE depletion aligns with a recent study reporting that depletion of cystathionine -synthase (CBS), another enzyme capable of supporting persulfide biosynthesis, promotes ferroptosis in breast cancer cells

In some cell types reduced ATP levels have been found to promote oxidative stress 50