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Structural information and biochemical experiments have been used to aid rational drug design to improve existing drugs and develop new treatments for the disease

The ethyl acetate plasmolysis method usually involves mixing of yeast cell suspension with ethyl acetate, adjustment of pH and temperature, further mixing for about 48 h, centrifugation, and finally analysis of the extract [8]

Requirement of BMP-2-induced phosphatidylinositol 3-kinase and Akt serine/threonine kinase in osteoblast differentiation and Smad-dependent BMP-2 gene transcription