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While the Clade 2 GS enzyme tested clearly has a slower rate of canonical enzyme activity than the Clade 1 GS, it can nevertheless synthesise glutathione: It has a high affinity for the -EC dipeptide substrate, and a preference for glycine as the terminal amino acid despite a lack of conservation in the two residues that apparently contribute to this specificity

doi: 10.1074/jbc.M104746200 128 QiaoLLuoGG

Andrs CMC, Prez de la Lastra JM, Juan CA, Plou FJ, Prez-Lebea E

It also activates mitochondrial-mediated apoptosis through downregulation of anti-apoptotic proteins (Bcl-2, survivin) and upregulation of pro-apoptotic markers (Bax, Bid, cytochrome c, and caspases 3, 8, and 9) (Ren et al., 2015

Fourteen days after initiation of treatment, a single local injection of the CFe/CP+Cis hydrogel (Group G6) achieved sustained tumor growth inhibition