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doi: 10.1111/j.1600-0897.2010.00852.x 95 GlebezdinaNSNekrasovaIVOlinaAASadykovaGKKuklinaEM

An increase in tonus of 0.5 mN was used as threshold for vasoconstriction because this difference is larger than the variation observed in the basal tonus of aorta rings

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Thus, the transition from crystal deposition to acute inflammation can be conceptualized as a two-hit process: First hit: MSU crystal deposition on cartilage surfaces activates synovial fibroblasts to release CCL2/MCP-1, recruiting monocytes and establishing subclinical inflammation