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Five days later, mice were randomized to receive carboplatin (50 mg/kg) ( n = 60 mice, 15 DIO shGPX4, 15 control shGPX4, 15 DIO shScramble, 15 control shScramble) or a vehicle control ( n = 60 mice, 15 DIO shGPX4, 15 control shGPX4, 15 DIO shScramble, 15 control shScramble), once weekly via intraperitoneal injection until average tumor size in any treatment group reached a volume of 1500 mm [3]