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-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 0.001 0.01 0.1 1 Difference in Absorbance @ 200 nm Concentration of Reduced Glutathione (mM) Binding of Opioids to Reduced Glutathione Ascorbic acid Naloxone Morphine Met-Enkephalin Methadone Dehydro-Asc Acid Figure 2

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Statements Author contributions EK: Writing review & editing, Writing original draft, Project administration, Funding acquisition, Formal analysis