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For standard cell culture, cells were grown in DMEM containing 25 mM glucose and 4 mM glutamine, and supplemented with 10% FBS, 1% penicillin/streptomycin, and prophylactic doses of plasmocin (Life Technologies, MPP-01-03) to prevent mycolplasma infection

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In PK deficiency, 2,3-BPG is increased as a result of the metabolic block at the PK step and of retrograde accumulation of products of glycolysis

GDF15 restrains myocardial ischemia-reperfusion injury through inhibiting GPX4 mediated ferroptosis

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