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At the end of the incubation, the mitochondrial membranes were washed with an excess of ice-cold 50mM phosphate buffer, pH 7.4, collected by centrifugation at 4C and resuspended in the same buffer

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Although beneficial in tumor cells, GPX4 inhibition can also trigger ferroptosis-induced death of immune cells in the tumor microenvironment leading to ineffective antitumor response